A Guide to Applied Molecular Testing
Author: William B. Coleman,Gregory J. Tsongalis
Publisher: Academic Press
Diagnostic Molecular Pathology: A Guide to Applied Molecular Testing is organized around disease types (genetic disease, infectious disease, neoplastic disease, among others). In each section, the authors provide background on disease mechanisms and describe how laboratory testing is built on knowledge of these mechanisms. Sections are dedicated to general methodologies employed in testing (to convey the concepts reflected in the methods), and specific description of how these methods can be applied and are applied to specific diseases are described. The book does not present molecular methods in isolation, but considers how other evidence (symptoms, radiology or other imaging, or other clinical tests) is used to guide the selection of molecular tests or how these other data are used in conjunction with molecular tests to make diagnoses (or otherwise contribute to clinical workup). In addition, final chapters look to the future (new technologies, new approaches) of applied molecular pathology and how discovery-based research will yield new and useful biomarkers and tests. Diagnostic Molecular Pathology: A Guide to Applied Molecular Testing contains exercises to test readers on their understanding of how molecular diagnostic tests are utilized and the value of the information that can be obtained in the context of the patient workup. Readers are directed to an ancillary website that contains supplementary materials in the form of exercises where decision trees can be employed to simulate actual clinical decisions. Focuses on the menu of molecular diagnostic tests available in modern molecular pathology or clinical laboratories that can be applied to disease detection, diagnosis, and classification in the clinical workup of a patient Explains how molecular tests are utilized to guide the treatment of patients in personalized medicine (guided therapies) and for prognostication of disease Features an ancillary website with self-testing exercises where decision trees can be employed to simulate actual clinical decisions Highlights new technologies and approaches of applied molecular pathology and how discovery-based research will yield new and useful biomarkers and tests
Author: Mary Louise Turgeon
Publisher: Elsevier Health Sciences
Immunology & Serology in Laboratory Medicine - E-Book
Author: Mohammad A Vasef,Aaron Auerbach
Publisher: Elsevier Health Sciences
This volume in the highly regarded Diagnostic Pathology series provides a clearer understanding of molecular pathology as it relates to the transformation and pathogenesis of cancer. Diagnostic Pathology: Molecular Oncology is the first reference to apply a practical and clinical approach to examining how molecular mutations affect common medical diseases. Additionally, it identifies the relevant and appropriate molecular tests to perform for a complete work-up in the era of molecular-targeted therapies. Easy to use and packed with outstanding illustrations, this is an essential resource for all pathologists or clinicians who need to keep up with the ever-evolving field of molecular medicine. Up-to-date information on a variety of topics Next-generation sequencing for determining gene mutations Diagnostic use of soft tissue mutations Use of solid tissue mutations such as EGFR, BRAF, and KRAS for diagnosis and treatment of different carcinomas and melanoma Interpreting newly found mutations in hematopathology such as MYD88 mutations Understanding bioinformatics Guidance on selecting the right test(s) at the right time for the right patient Comparisons of molecular testing methods (i.e., FISH testing vs. cytogenetics) More than 2,000 images including H&E stains, immunostains, and FISH images
Author: Eberhard Passarge
Author: Nader Rifai,A. Rita Horvath,Carl T. Wittwer,Jason Park
Principles and Applications of Molecular Diagnostics serves as a comprehensive guide for clinical laboratory professionals applying molecular technology to clinical diagnosis. The first half of the book covers principles and analytical concepts in molecular diagnostics such as genomes and variants, nucleic acids isolation and amplification methods, and measurement techniques, circulating tumor cells, and plasma DNA; the second half presents clinical applications of molecular diagnostics in genetic disease, infectious disease, hematopoietic malignancies, solid tumors, prenatal diagnosis, pharmacogenetics, and identity testing. A thorough yet succinct guide to using molecular testing technology, Principles and Applications of Molecular Diagnostics is an essential resource for laboratory professionals, biologists, chemists, pharmaceutical and biotech researchers, and manufacturers of molecular diagnostics kits and instruments. Explains the principles and tools of molecular biology Describes standard and state-of-the-art molecular techniques for obtaining qualitative and quantitative results Provides a detailed description of current molecular applications used to solve diagnostics tasks
Category: Histology, Pathological
Author: J. Suso Platero
Publisher: John Wiley & Sons
Covers powerful new tools for drug development Molecular pathology offers tools and techniques that can greatly enhance the drug discovery and development process, helping to make the promises of personalized medicine a reality. Molecular Pathology in Drug Discovery and Development provides an unmatched guide to this cutting-edge discipline and its applications to pharmaceutical science. With contributions from leading lights in drug discovery, drug development, and molecular pathology balanced by a consistent editorial approach, this reference offers both an overview of molecular pathology and a close look at the methods as they are applied to the process of drug discovery and development. Presented as steps in the drug development process, the coverage includes the use of molecular pathology to: Identify and validate new drug candidates Enhance transcriptional profiling to better find and validate biomarkers Assess toxicology Employ toxicogenomics to identify genes relevant to the safety of compounds Identify correct doses for different drugs Identify patients for treatment Develop molecular therapies Further the new techniques of Immunohistochemistry and Immunofluorescence With many tests and treatments already working today, drug research and development using molecular pathology has shown itself an extremely fruitful area. Molecular Pathology in Drug Discovery and Development gives practitioners an up-to-date resource on this highly active discipline and its role in furthering pharmaceutical research.
Author: Sunil Badve,Yesim Gökmen-Polar
The complex landscape of breast cancer requires distinct strategies for the management of various molecular subtypes of this disease. Rapid advances in the field of molecular biology have been bewildering for those involved in its study and management. “Molecular Pathology of Breast Cancer” aims to close this knowledge gap by discussing comprehensively the evolution, biological basis and clinical applications with a focus on the “what, when, and how” of the most significant molecular markers known to date. These markers are evaluated in the context of genomic, transcriptomic and proteomic profiles, which is integral to the practice of precision medicine. The application of next generation sequencing (NGS) has provided new insights in the regulation of genomic and transcriptomic structure and function. Alterations in DNA such as mutations and single nucleotide polymorphisms (SNPs) have been correlated with outcomes and provide for novel therapeutic approaches. These NGS analyses have also revealed the extensive contributions of epigenetic mechanisms such as histone modifications, non-coding RNA and alternative splicing. All of these changes together contribute to alterations in proteome. Newer assays that allow greater stability and analytical consistency are emerging. These alterations in tumor profiles can be also now detected by imaging techniques. The heterogeneity of both tumor and tumor microenvironment, an inevitable reality, is discussed in detail with particular focus on cancer stem cells and immune signaling. A chapter is dedicated to the emerging technology of “liquid biopsy”, which opens a novel approach for “continuous” monitoring of cancer that might be superior to conventional diagnostics, “Molecular Pathology of Breast Cancer” provides a quick and easy, not to mention essential, tour for clinicians, pathologists and scientists who are seeking to understand the integration of molecular biology into the diagnosis, prognosis and management of breast cancer.
Diagnostik makroskopischer, histologischer und radiologischer Strukturveränderungen des Skeletts
Author: Claus-Peter Adler
Dieses interdisziplinäre Nachschlagewerk bietet Ihnen das Fundament für Ihre exakte Diagnose und gezielte Therapie-Planung. o Sie erhalten einen praxisorientierten Überblick über alle modernen und etablierten Diagnostik-Verfahren. o Didaktisch übersichtlich stehen die jeweiligen radiologischen und pathologisch-anatomischen Strukturen dem Text gegenüber. o Kurzgefaßte Therapievorschläge ergänzen die diagnostischen Richtlinien. => Das aktuelle Nachschlagewerk für die multidisziplinäre Zusammenarbeit von Orthopäden, Radiologen, Rheumatologen und Pathologen. Schnell * präzise * praxisgerecht
Author: John M. S. Bartlett,Abeer Shaaban,Fernando Schmitt
Publisher: Cambridge University Press
Practical overview of current molecular techniques and their applications in each organ system, for practising and trainee pathologists.
Molecular Genetics; Lung and Breast Carcinomas
Author: M. A. Hayat
The various cell types have traditionally been recognized and classified according to their appearance in the light microscope following the process of fixing, processing, sectioning, and staining tissues that is known as histology. Classical histology has been augmented by immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system. Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today, ensuring a wide audience for this book. The only book available that translates molecular genetics into cancer diagnosis Provides the readers with tools necessary to perform and optimize sensitive, powerful techniques, including immunohistochemistry and fluorescence in situ hybridization, used in tumor diagnosis Written by experts in this field, the book provides theoretical considerations as well as practical approaches to carry out effectively these techniques Offers suggestions, tips, cautions, and guidelines to avoid artifacts and misdiagnosis Introduces new techniques to detect genes and proteins involved in the initiation and progression of cancer Covers the latest developments and a wide range of applications to the detection of antigens and single-copy DNA and RNA Written in a uniform format, each chapter includes Introduction, Materials required, step-by-step detailed Methods, Results, Discussion, and comprehensive up-to-date References
Author: John E. Niederhuber,James O. Armitage,James H Doroshow,Michael B. Kastan,Joel E. Tepper
Publisher: Elsevier Health Sciences
Practical and clinically focused, Abeloff’s Clinical Oncology is a trusted medical reference book designed to capture the latest scientific discoveries and their implications for cancer diagnosis and management of cancer in the most accessible manner possible. Abeloff’s equips everyone involved - from radiologists and oncologists to surgeons and nurses - to collaborate effectively and provide the best possible cancer care. Consult this title on your favorite e-reader, conduct rapid searches, and adjust font sizes for optimal readability. Select the most appropriate tests and imaging studies for cancer diagnosis and staging of each type of cancer, and manage your patients in the most effective way possible by using all of the latest techniques and approaches in oncology. Enhance your understanding of complex concepts with a color art program that highlights key points and illustrates relevant scientific and clinical problems. Stay at the forefront of the latest developments in cancer pharmacology, oncology and healthcare policy, survivorship in cancer, and many other timely topics. See how the most recent cancer research applies to practice through an increased emphasis on the relevance of new scientific discoveries and modalities within disease chapters. Streamline clinical decision making with abundant new treatment and diagnostic algorithms as well as concrete management recommendations. Take advantage of the collective wisdom of preeminent multidisciplinary experts in the field of oncology, including previous Abeloff’s editors John E. Niederhuber, James O. Armitage, and Michael B. Kastan as well as new editors James H. Doroshow from the National Cancer Institute and Joel E. Tepper of Gunderson & Tepper: Clinical Radiation Oncology. Quickly and effortlessly access the key information you need with the help of an even more user-friendly, streamlined format. Access the complete contents anytime, anywhere at Expert Consult, and test your mastery of the latest knowledge with 500 online multiple-choice review questions.
A Volume in the Foundations in Diagnostic Pathology Series
Author: Raymond R. Tubbs,Mark H. Stoler
Publisher: Elsevier Health Sciences
This volume in the Foundations in Diagnostic Pathology Series packs today's most essential cell and tissue base molecular pathology into a compact, high-yield format! It focuses on the state of the art in practical validated molecular diagnostics as applied across the fields of surgical pathology and cytology. With an emphasis on current, clinically valid, and diagnostically important applications today and in the near future, you can be assured you’re getting the most up-to-date, authoritative coverage available. Its pragmatic, well-organized approach, nearly 250 full-color illustrations, and at-a-glance boxes and tables make the information you need easy to access. Practical and affordable, this resource is ideal for study and review as well as everyday clinical practice! Offers detailed discussions on today’s technologies to help you select the best test for case evaluation. Presents recognized molecular pathologists who convey the most current information, keeping you on the cusp of your field. Features nearly 250 full-color illustrations that present important pathologic features, enabling you to form a differential diagnosis and compare your findings with actual cases. Uses a consistent, user-friendly format, including at-a-glance boxes and tables for easy reference.
Author: Liang Cheng,David Y. Zhang
Publisher: Springer Science & Business Media
This volume presents a useful and up-to-date handbook containing information relevant to the clinical practice of molecular genetic pathology. It features organized, detailed text on specific molecular genetic techniques. The volume provides a unique reference for the practicing pathologist and medical geneticist, as well as a review book for residents and fellows in training in pathology, medical genetics and molecular genetic pathology.
Author: Richard A. McPherson,Matthew R. Pincus
Publisher: Elsevier Health Sciences
Recognized as the definitive book in laboratory medicine since 1908, Henry’s Clinical Diagnosis and Management by Laboratory Methods, edited by Richard A. McPherson, MD and Matthew R. Pincus, MD, PhD, is a comprehensive, multidisciplinary pathology reference that gives you state-of-the-art guidance on lab test selection and interpretation of results. Revisions throughout keep you current on the latest topics in the field, such as biochemical markers of bone metabolism, clinical enzymology, pharmacogenomics, and more! A user-friendly full-color layout puts all the latest, most essential knowledge at your fingertips. Update your understanding of the scientific foundation and clinical application of today's complete range of laboratory tests. Get optimal test results with guidance on error detection, correction, and prevention as well as cost-effective test selection. Reference the information you need quickly and easily thanks to a full-color layout, many new color illustrations and visual aids, and an organization by organ system. Master all the latest approaches in clinical laboratory medicine with new and updated coverage of: the chemical basis for analyte assays and common interferences; lipids and dyslipoproteinemia; markers in the blood for cardiac injury evaluation and related stroke disorders; coagulation testing for antiplatelet drugs such as aspirin and clopidogrel; biochemical markers of bone metabolism; clinical enzymology; hematology and transfusion medicine; medical microbiology; body fluid analysis; and many other rapidly evolving frontiers in the field. Effectively monitor the pace of drug clearing in patients undergoing pharmacogenomic treatments with a new chapter on this groundbreaking new area. Apply the latest best practices in clinical laboratory management with special chapters on organization, work flow, quality control, interpretation of results, informatics, financial management, and establishing a molecular diagnostics laboratory. Confidently prepare for the upcoming recertification exams for clinical pathologists set to begin in 2016.
Techniques and Applications
Author: Gerhard W. Hacker,Raymond R. Tubbs
Publisher: CRC Press
Molecular Morphology in Human Tissues: Techniques and Applications presents the most advanced molecular morphological techniques to date. This integrated approach to molecular morphology provides powerful analytical and diagnostic tools at the genome level, making the diagnosis and management of cancer, viral infections, and other diseases more precise and reliable. The pioneers of these techniques contribute step-by-step protocols with tips, guidelines, and other suggestions from their own notebooks, enabling experienced scientists as well as beginners to apply the most progressive detection methods available and to obtain optimal results.
Author: D. Hunter Best,Jeffrey J. Swensen
Publisher: Springer Science & Business Media
Genetic testing has become commonplace, and clinicians are frequently able to use knowledge of an individual’s specific genetic differences to guide their course of action. Molecular Genetics and Personalized Medicine highlights developments that have been made in the field of molecular genetics and how they have been applied clinically. It will serve as a useful reference for physicians hoping to better understand the role of molecular medicine in clinical practice. In addition, it should also prove to be an invaluable resource for the basic scientist that wants to better understand how advances in the laboratory are being moved from the bench to the bedside. All chapters are written by experts in their fields and include the most up to date medical information. The authors simplify complex genetic concepts and focus on practical patient related issues. The book will be of great value to pathologists, hematologists/oncologists, clinical geneticists, high-risk obstetricians, general practitioners, and physicians in all other medical specialties who utilize genetic testing to direct therapy.
Author: Brian Freeman
Publisher: McGraw Hill Professional
Online 3rd edition inlcudes only selected chapters as of 1/10/2014.
Author: Carl A. Burtis,Edward R. Ashwood,David E. Bruns
Publisher: Elsevier Health Sciences
As the definitive reference for clinical chemistry, Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 5th Edition offers the most current and authoritative guidance on selecting, performing, and evaluating results of new and established laboratory tests. Up-to-date encyclopedic coverage details everything you need to know, including: analytical criteria for the medical usefulness of laboratory procedures; new approaches for establishing reference ranges; variables that affect tests and results; the impact of modern analytical tools on lab management and costs; and applications of statistical methods. In addition to updated content throughout, this two-color edition also features a new chapter on hemostasis and the latest advances in molecular diagnostics. Section on Molecular Diagnostics and Genetics contains nine expanded chapters that focus on emerging issues and techniques, written by experts in field, including Y.M. Dennis Lo, Rossa W.K. Chiu, Carl Wittwer, Noriko Kusukawa, Cindy Vnencak-Jones, Thomas Williams, Victor Weedn, Malek Kamoun, Howard Baum, Angela Caliendo, Aaron Bossler, Gwendolyn McMillin, and Kojo S.J. Elenitoba-Johnson. Highly-respected author team includes three editors who are well known in the clinical chemistry world. Reference values in the appendix give you one location for comparing and evaluating test results. NEW! Two-color design throughout highlights important features, illustrations, and content for a quick reference. NEW! Chapter on hemostasis provides you with all the information you need to accurately conduct this type of clinical testing. NEW! Six associate editors, Ann Gronowski, W. Greg Miller, Michael Oellerich, Francois Rousseau, Mitchell Scott, and Karl Voelkerding, lend even more expertise and insight to the reference. NEW! Reorganized chapters ensure that only the most current information is included.