Flow Cytometry in Clinical Diagnosis

Author: David E. Keren

Publisher: American Society of Clinical Oncology

ISBN: 9780891895480

Category: Flow cytometry

Page: 384

View: 1332

The new edition promises a completely current overview of this increasingly commonplace technology. With every chapter either completely reworked or significantly updated to include the latest developments and new contributions from 10 new international authorities, this will be the go-to resource on clinical flow cytometry for years to come. More than that it promises to deliver a uniquely engaging learning experience with a bonus CD filled with real-world cases and interpretations. Drs. Carey and McCoy bring the new edition of 'Flow Cytometry in Clinical Diagnosis' alive with the bonus CD, which includes data from actual flow analysis and patient samples for 39 cases.

Zelluläre Diagnostik

Grundlagen, Methoden und klinische Anwendungen der Durchflusszytometrie ; 163 Tabellen

Author: Ulrich Sack,A. Tarnok,G. Rothe

Publisher: Karger Medical and Scientific Publishers

ISBN: 3805579284

Category: Medical

Page: 1111

View: 7576

Das vorliegende Buch ist die einzige umfassende, deutschsprachige Darstellung der Durchflusszytometrie und ihrer Anwendungen. Während einige Techniken der Immunphänotypisierung mit der Durchflusszytometrie bereits zu Routineverfahren in der Laboratoriumsmedizin geworden sind, beginnen sich neue Methoden der funktionellen Charakterisierung von Zellen, der Analyse seltener Zellen und der Diagnostik aus komplexen Materialien erst zu etablieren. Durch neue Ansätze wie die objektträgergebundene Zytometrie wird der Einsatz dieser Verfahren weiter zunehmen. Multiparameteranalysen und Miniaturisierung sind weitere Schritte zur Verbesserung der Analytik. Das Buch bietet eine umfassende, fundierte und detaillierte Zusammenstellung aller Aspekte der Durchflusszytometrie in Forschung und Klinik. Neueinsteigern gibt es eine fundierte Einführung, fortgeschrittenen Anwendern spezielle Protokolle und Interpretationshilfen.

Molecular Diagnostics

For the Clinical Laboratorian

Author: William B. Coleman,Gregory J. Tsongalis

Publisher: Springer Science & Business Media

ISBN: 1592599281

Category: Medical

Page: 567

View: 9368

Accompanying CD-ROM contains ... "a companion eBook version of Molecular diagnostics : for the clinical laboratorian, Second edition ... for downloading and use in the reader's PC or PDA." -- p. [4] of cover.

Flow Cytometry in Drug Discovery and Development

Author: Virginia Litwin,Philip Marder

Publisher: John Wiley & Sons

ISBN: 0470922788

Category: Medical

Page: 360

View: 2769

This book covers the unique application of flow cytometry in drug discovery and development. The first section includes two introductory chapters, one on flow cytometry and one on biomarkers, as well as a chapter on recent advances in flow cytometry. The second section focuses on the unique challenges and added benefits associated with the use of flow cytometry in the drug development process. The third section contains a single chapter presenting an in depth discussion of validation considerations and regulatory compliance issues associated with drug development.

Henry's Clinical Diagnosis and Management by Laboratory Methods E-Book

Author: Richard A. McPherson,Matthew R. Pincus

Publisher: Elsevier Health Sciences

ISBN: 0323413153

Category: Medical

Page: 1700

View: 7664

Recognized as the definitive reference in laboratory medicine since 1908, Henry's Clinical Diagnosis continues to offer state-of-the-art guidance on the scientific foundation and clinical application of today's complete range of laboratory tests. Employing a multidisciplinary approach, it presents the newest information available in the field, including new developments in technologies and the automation platforms on which measurements are performed. Provides guidance on error detection, correction, and prevention, as well as cost-effective test selection. Features a full-color layout, illustrations and visual aids, and an organization based on organ system. Features the latest knowledge on cutting-edge technologies of molecular diagnostics and proteomics. Includes a wealth of information on the exciting subject of omics; these extraordinarily complex measurements reflect important changes in the body and have the potential to predict the onset of diseases such as diabetes mellitus. Coverage of today's hottest topics includes advances in transfusion medicine and organ transplantation; molecular diagnostics in microbiology and infectious diseases; point-of-care testing; pharmacogenomics; and the microbiome. Toxicology and Therapeutic Drug Monitoring chapter discusses the necessity of testing for therapeutic drugs that are more frequently being abused by users.

Practical Flow Cytometry

Author: Howard M. Shapiro

Publisher: John Wiley & Sons

ISBN: 0471434035

Category: Science

Page: 736

View: 1276

From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.

Practical Flow Cytometry in Haematology

100 Worked Examples

Author: Mike Leach,Mark Drummond,Allyson Doig,Pam McKay,Bob Jackson,Barbara J. Bain

Publisher: John Wiley & Sons

ISBN: 1118746899

Category: Medical

Page: 416

View: 3212

The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors’ institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis. The reader will review May−Grünwald−Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference. Practical Flow Cytometry in Haematology: 100 Worked Examples: Provides a practical, example-based resource for flow cytometry Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care Includes both malignant and benign conditions Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207) Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.

Flow Cytometry in Neoplastic Hematology

Morphologic-immunophenotypic Correlation

Author: Wojciech Gorczyca

Publisher: Informa Healthcare

ISBN: 9781841845654

Category: Medical

Page: 266

View: 9427

Key features of this highly illustrated and practical guide include comprehensive coverage of the aspects relevant for clinical diagnosis, an explanation of the general parameters useful in diagnosis with flow cytometry, correlation with color histomorphological findings throughout, and a systematic approach from basic cases to complicated problem areas. All hematopathologists and neoplastic hematologists will find this an important resource for keeping up to date with developments in clinical practice.

Flow Cytometry of Hematological Malignancies

Author: Claudio Ortolani

Publisher: John Wiley & Sons

ISBN: 144433588X

Category: Medical

Page: 311

View: 534

Flow Cytometry of Hematological Malignancies contains an array of graphical outputs produced by the technique in the study of the most (and the least) common diseases. The images included allow you to compare your own results with a third party reference pattern. There is a detailed description of the main leukocyte antigens, together with a description of their distribution amongst normal and abnormal blood cells. The book also provides a comprehensive description of the phenotype of every neoplastic blood disease recorded in the WHO classification system, including all the instructions needed to recognise and classify even the least common entity. Designed to be practical, the book is perfect for quick consultation and is divided into two main sections. Section I deals with the direct object of immunophenotyping, and Section II deals with the ultimate target of the analysis. More than 50 antigens are covered and every antigen is dealt with in three main parts: general features, cytometric features and practical hints. This authoritative and state–of–the–art reference will be invaluable for clinicians directly involved in the diagnosis and analysis of hematological diseases, including hematologists, hematopathologists, oncologists, pathologists and technicians working in diagnostic laboratories.

Flow Cytometry Principles for Clinical Laboratory Practice

Quality Assurance for Quantitative Immunophenotyping

Author: Marilyn A. Owens,Michael R. Loken

Publisher: University of Texas Press

ISBN: 9780471021766

Category: Medical

Page: 224

View: 2650

FLOW CYTOMETRY PRINCIPLES FOR CLINICAL LABORATORY PRACTICE Quality Assurance for Quantitative Immunophenotyping Marilyn A. Owens and Michael R. Loken Flow Cytometry Principles for Clinical Laboratory Practice is an invaluable benchtop reference and introduction for first-time users of flow cytometry in clinical diagnostic laboratories. This manual provides authoritative coverage of accepted methods, quality controls, and standards for the cytometric analysis of clinical samples with particular relevance to CD4+ lymphocytes. CD4+ lymphocyte determination by immunophenotyping is the standard method for evaluating the status of HIV infection, and the single most frequent application of flow cytometry in a diagnostic setting. Flow Cytometry Principles for Clinical Laboratory Practice will appeal to clinical laboratory technologists, medical practitioners, and cytometry lab directors in their role as instructors of new personnel. In addition, it is a valuable resource for clinical and biomedical researchers, establishing quantitative cytometric systems for studies in immunology and hematology.

Rosai and Ackerman's Surgical Pathology E-Book

Author: Juan Rosai

Publisher: Elsevier Health Sciences

ISBN: 032308804X

Category: Medical

Page: 2892

View: 4455

Rosai and Ackerman’s Surgical Pathology delivers the authoritative guidance you need to overcome virtually any challenge in surgical pathology. Recognized globally for his unmatched expertise, preeminent specialist Juan Rosai, MD brings you state-of-the-art coverage of the latest advancements in immunohistochemistry, genetics, molecular biology, prognostic/predictive markers, and much more - equipping you to effectively and efficiently diagnose the complete range of neoplastic and non-neoplastic entities. Efficiently review the clinical presentation, gross and microscopic features, ultrastructural and immunohistochemical findings, differential diagnosis, therapy, and prognosis for virtually every pathologic entity. Compare your findings to more than 3,300 outstanding illustrations that capture the characteristic presentation of every type of lesion. Avoid diagnostic pitfalls using Dr. Rosai's expert observations on what to look for, what to be careful about, and which presentations can be misleading. Find quick answers on tumor staging, quality control procedures, and the handling of gross specimens through valuable appendices. Make optimal use of all the very latest advances including our increased understanding of the genetic basis of inherited and acquired disease, the newest molecular genetic and immunohistochemical techniques, and the most recent WHO disease classification schemes.

Cellular Diagnostics

Basic Principles, Methods and Clinical Applications of Flow Cytometry

Author: Ulrich Sack,Attila Tárnok,Gregor Rothe

Publisher: Karger Medical and Scientific Publishers

ISBN: 3805585551

Category: Medical

Page: 738

View: 979

This book is the updated English version of the 2006 German bestseller Zellulare Diagnostik, a comprehensive presentation of flow cytometry and its applications. While some techniques of immunophenotyping by flow cytometry already are routine procedures in the laboratory, new methods for the functional characterization of cells, the analysis of rare cells, and the diagnosis of complex materials have only begun to win wide recognition. New approaches such as slide-based cytometry will lead to an increase in the use of cytometric techniques. Multiparameter approaches will further improve analysis. The book provides a comprehensive and detailed compilation of all aspects of flow cytometry in research and the clinic. For newcomers it offers a thorough introduction, for advanced users, specific protocols and interpretation assistance.

Abeloff's Clinical Oncology E-Book

Author: John E. Niederhuber,James O. Armitage,James H Doroshow,Michael B. Kastan,Joel E. Tepper

Publisher: Elsevier Health Sciences

ISBN: 1455728810

Category: Medical

Page: 2224

View: 446

Practical and clinically focused, Abeloff’s Clinical Oncology is a trusted medical reference book designed to capture the latest scientific discoveries and their implications for cancer diagnosis and management of cancer in the most accessible manner possible. Abeloff’s equips everyone involved - from radiologists and oncologists to surgeons and nurses - to collaborate effectively and provide the best possible cancer care. Consult this title on your favorite e-reader, conduct rapid searches, and adjust font sizes for optimal readability. Select the most appropriate tests and imaging studies for cancer diagnosis and staging of each type of cancer, and manage your patients in the most effective way possible by using all of the latest techniques and approaches in oncology. Enhance your understanding of complex concepts with a color art program that highlights key points and illustrates relevant scientific and clinical problems. Stay at the forefront of the latest developments in cancer pharmacology, oncology and healthcare policy, survivorship in cancer, and many other timely topics. See how the most recent cancer research applies to practice through an increased emphasis on the relevance of new scientific discoveries and modalities within disease chapters. Streamline clinical decision making with abundant new treatment and diagnostic algorithms as well as concrete management recommendations. Take advantage of the collective wisdom of preeminent multidisciplinary experts in the field of oncology, including previous Abeloff’s editors John E. Niederhuber, James O. Armitage, and Michael B. Kastan as well as new editors James H. Doroshow from the National Cancer Institute and Joel E. Tepper of Gunderson & Tepper: Clinical Radiation Oncology. Quickly and effortlessly access the key information you need with the help of an even more user-friendly, streamlined format. Access the complete contents anytime, anywhere at Expert Consult, and test your mastery of the latest knowledge with 500 online multiple-choice review questions.

Management of Cancer in the Older Patient E-Book

Author: Arash Naeim,David Reuben,Patricia Ganz

Publisher: Elsevier Health Sciences

ISBN: 1455723134

Category: Medical

Page: 312

View: 2942

Management of Cancer in the Older Patient, by Drs. Arash Naeim, David Reuben, and Patricia Ganz, offers the help you need to effectively diagnose, refer, and manage cancer in geriatric patients. You’ll see how to provide effective cancer screening; refer your patients to the right oncologist; deal with comorbidities, frailties, and other complications; navigate end-of-life issues; and much more. A templated, user-friendly format makes it easy to find and apply the answers you need. See how to best manage geriatric cancer patients with help from leading specialists in both geriatrics and oncology Make informed decisions as to when to refer patients to specialists. Provide the supportive care your patients and their families need on issues such as such as mental health, pain, fatigue, nausea, insomnia. Be prepared to help cancer survivors navigate their after-treatment care including adjuvant therapy, side effects, second cancers, quality of life, and other concerns. Offer accurate guidance on ethical issues like competency, end of life, hospice, the role of the caregiver, and more.

Flow Cytometry in Hematopathology

A Visual Approach to Data Analysis and Interpretation

Author: Doyen T. Nguyen,Lawrence W. Diamond,Raul C. Braylan

Publisher: Springer Science & Business Media

ISBN: 9781597451628

Category: Medical

Page: 344

View: 9153

The second edition of this volume reflects the recent advances in the FCM analysis of hematopoietic disorders. The chapters have been revised to incorporate new text and figures. The volume is aimed at hematopathologists, hematologists, pathologists, and laboratory technicians.

Flow Cytometry

Principles and Applications

Author: Marion G. Macey

Publisher: Springer Science & Business Media

ISBN: 1597454516

Category: Medical

Page: 294

View: 904

Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.