Principles of Fluorescence Spectroscopy

Author: Joseph R. Lakowicz

Publisher: Springer Science & Business Media

ISBN: 9780387463124

Category: Science

Page: 954

View: 5740

The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

Introduction to Fluorescence

Author: David M. Jameson

Publisher: Taylor & Francis

ISBN: 1439806047

Category: Science

Page: 313

View: 337

The phenomenon known as fluorescence is now widely used in the chemical and life sciences largely due to the development of highly sophisticated fluorescent probe chemistries and the commercial availability of these probes as well as the development of novel microscopy approaches. Introduction to Fluorescence helps readers acquire a sound understanding of basic fluorescence theory and practice. It describes general principles in a straightforward way and uses examples from a variety of disciplines to demonstrate them. In color throughout, the book takes readers through the history of important discoveries to the most current advances. It introduces the fundamentals of the fluorescence phenomenon and gives detailed examples of fluorescence applications in the molecular life sciences, including biochemistry, biophysics, clinical chemistry and diagnostics, pharmaceutical science, and cell and molecular biology. The author presents the basic theories underlying the applications and offers in-depth information on practical aspects. Along with a list of references in each chapter, the text incorporates more than 250 figures that clearly illustrate the concepts and gives the chemical structures of the most widely used fluorescent molecules. In addition, the appendix provides a "Rogue’s Gallery" of the most common errors and pitfalls to avoid.

Molecular Fluorescence

Principles and Applications

Author: Bernard Valeur,Mário Nuno Berberan-Santos

Publisher: John Wiley & Sons

ISBN: 3527650024

Category: Science

Page: 500

View: 9960

This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.

Fluorescence Microscopy

Author: F. W. D. Rost

Publisher: Cambridge University Press

ISBN: 9780521410885

Category: Science

Page: 473

View: 3779

Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts, for example in living cells. This magnificent new work provides comprehensive cover of all aspects of fluorescence microscopy - including instrumentation, applications, and the history of the technique. The first volume deals with instrumentation and techniques for fluorescence microscopy, and includes a chapter on quantification and scanning. The second volume deals with the applications of fluorescence microscopy in many fields. It includes information on autofluorescence, and an invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations.

Fluorescence Microscopy and Fluorescent Probes

Author: Jan Slavík

Publisher: Springer Science & Business Media

ISBN: 9780306460210

Category: Science

Page: 272

View: 9312

The April 1997 conference held in Prague attracted the cream of primarily European and Russian researchers (with a handful from the US, primarily from the U. of Maryland School of Medicine) to the burgeoning biological and medical applications of innovative optical technology, particularly laser con


Gems and Minerals Under Ultraviolet Light

Author: Manuel Robbins

Publisher: N.A


Category: Nature

Page: 374

View: 2213

The complete source for understanding and locating fluorescent minerals. Documents all major locations in the U.S. and throughout the world. One of very few publications on this subject. Includes experiments for home and school use.

Fluorescence Applications in Biotechnology and Life Sciences

Author: Ewa M. Goldys

Publisher: John Wiley & Sons

ISBN: 0470083700

Category: Science

Page: 367

View: 8125

Fluorescence Applications in Biotechnology and the Life Sciences Edited by Ewa M. Goldys A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences Fluorescence Applications in Biotechnology and the Life Sciences is the first reference in this important subject area to focus specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It is designed to raise awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environ-mental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book will appeal to postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.

Handbook of Fluorescence Spectroscopy and Imaging

From Ensemble to Single Molecules

Author: Markus Sauer,Johan Hofkens,J?rg Enderlein

Publisher: John Wiley & Sons

ISBN: 3527633529

Category: Science

Page: 280

View: 2006

Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

New Trends in Fluorescence Spectroscopy

Applications to Chemical and Life Sciences

Author: Bernard Valeur,Jean-Claude Brochon

Publisher: Springer Science & Business Media

ISBN: 364256853X

Category: Science

Page: 490

View: 3487

This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.

Applications of Fluorescence in Immunoassays

Author: Ilkka A. Hemmilä

Publisher: Wiley-Interscience


Category: Medical

Page: 343

View: 9464

Describes the basic prerequisities for a fluorometric immunoassay: the antibody, the immunological technology, the fluorescent probe, and the instrument. Provides a profile of the clinical applications of the various assay technologies. Topics include: background noise, the mechanization and automation of immunoassays, DNA-based specific binding assays, fluorescence immonosensors, multiparameter analyzing systems and more.



Author: Jennifer K. Dick

Publisher: University of Georgia Press

ISBN: 9780820326917

Category: Poetry

Page: 89

View: 1321

In Jennifer K. Dick's first book, Fluorescence, very real places--Paris, Massachusetts, Colorado, Iowa, Morocco--mix into the imagined, into Breughelian villages where there's "a persimmon in the corner knitting." These places are inhabited by varied but always very real bodies, stretching outward from their own edges and encountering, or engendering, a certain luminescence in the process. What happens when we exceed ourselves? When fragments of dream are lifted to the surface and through to something beyond? Clues, keys, indications--all that once seemed certain slips off into code. These poems use language to crack it.

Practical Fluorescence, Second Edition

Author: George G. Guilbault

Publisher: CRC Press

ISBN: 9780824783501

Category: Science

Page: 826

View: 2045

New edition (first, 1973) of an introduction to the principles and applications of all phases of luminescence spectroscopy. Contains (all rewritten) chapters on general aspects of luminescence, instrumentation, effects of molecular structure and environment, inorganic analysis, phosphorescence, fluo

Fluorescence Spectroscopy in Biology

Advanced Methods and their Applications to Membranes, Proteins, DNA, and Cells

Author: Martin Hof,Rudolf Hutterer,V. Fidler

Publisher: Springer Science & Business Media

ISBN: 3540270043

Category: Science

Page: 305

View: 4213

Optical Fluorescence Microscopy

From the Spectral to the Nano Dimension

Author: Alberto Diaspro

Publisher: Springer Science & Business Media

ISBN: 9783642151750

Category: Science

Page: 244

View: 6910

In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Reviews in Fluorescence 2008

Author: Chris D. Geddes

Publisher: Springer Science & Business Media

ISBN: 9781441912602

Category: Science

Page: 490

View: 7386

This volume serves as a comprehensive collection of current trends and emerging hot topics in the field of fluorescence spectroscopy. It summarizes the year’s progress in fluorescence and its applications as well as includes authoritative analytical reviews.

Principles and Applications of Fluorescence Spectroscopy

Author: Jihad Rene Albani

Publisher: John Wiley & Sons

ISBN: 0470691336

Category: Science

Page: 264

View: 8150

Fluorescence spectroscopy is an important investigational tool in many areas of analytical science, due to its extremely high sensitivity and selectivity. With many uses across a broad range of chemical, biochemical and medical research, it has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems with extremely high resolution. It is particularly heavily used in the pharmaceutical industry where it has almost completely replaced radiochemical labelling. Principles and Applications of Fluorescence Spectroscopy gives the student and new user the essential information to help them to understand and use the technique confidently in their research. By integrating the treatment of absorption and fluorescence, the student is shown how fluorescence phenomena arise and how these can be used to probe a range of analytical problems. A key element of the book is the inclusion of practical laboratory experiments that illustrate the fundamental points and applications of the technique.

Introduction to fluorescence microscopy

Author: J. S. Ploem,H. J. Tanke

Publisher: Oxford University Press

ISBN: 9780198564089

Category: Science

Page: 56

View: 1692

This handbook provides a thorough and detailed introduction to fluorescence microscopy by exploring three main areas of the field. First discussed are the physical principles of the phenomenon of fluorescence and fluorescence microscopy, including transmitted versus incident illumination. Next is a detailed description of various microscope parts, including lenses, filters, and light sources, and a discussion of the main applications of fluorescence microscopy, including immunofluorescence, microflurometry, and photomicrography. The final section enables the reader to solve the most common problems in fluorescence microscopy. Each chapter includes an up-to-date list of review articles and books for further reading, and there are illustrations throughout the text of typical microscopes and optical components. Biologists, students, and researchers of fluorescence microscopy will value this clear and indispensable guide to the field.


Fire Starter

Author: P. Anastasia

Publisher: P. Anastasia

ISBN: 0986256714

Category: Juvenile Fiction

Page: 262

View: 2350

Mortal Instruments meets The Tomorrow People. Book 1 of the amazon bestselling tetralogy, and a riveting coming-of-age tale, Fluorescence is engaging and unabashed—an urban fantasy unlike any other. It lives in her bloodstream. It’s unpredictable and could flare up anytime, exposing her secret. Alice was a normal teenager until a dying race of aliens chose her and a handful of others to preserve bioluminescent DNA known as Fluorescence. Now she and the others must hide their condition from the rest of the world, while trying to learn the truth behind the living light. In a unique genre all its own, Fluorescence is a striking blend of audacious young love, fantasy and sci-fi. Each full-length novel in the series is narrated by a different character, driving the story forward in a new and exciting way. It evolves from a quiet beginning into a gripping saga exploring the real-life limitations encountered while harboring a volatile secret. Novels in the complete Fluorescence series:Book 1: Fire StarterBook 2: ContagiousBook 3: FalloutBook 4: Lost Souls

Quantitative Fluorescence Microscopy

Author: F. W. D. Rost

Publisher: Cambridge University Press

ISBN: 9780521394222

Category: Medical

Page: 236

View: 920

Quantitative fluorescence microscopy is concerned with making measurements from fluorescent specimens in a fluorescence microscope, by measuring fluorescence emission from a defined area or areas of a specimen. This technique is most commonly used to determine the amount of some specific substance, such as DNA, in some particular area of a cell. But it has many other uses; for example, it can be used to identify certain substances in the cell by examining their fluorescence characteristics. This book is a complete guide to this technique for all biologists. It describes the principles and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. There is also a discussion of the exciting developments in confocal fluorescence microscopy which allows the three dimensional distribution of particular substances to be determined. Everyone presently using this technique, or wishing to start using it will need to read this book.