Introduction to Fluorescence

Author: David M. Jameson

Publisher: Taylor & Francis

ISBN: 1439806047

Category: Science

Page: 313

View: 4865

The phenomenon known as fluorescence is now widely used in the chemical and life sciences largely due to the development of highly sophisticated fluorescent probe chemistries and the commercial availability of these probes as well as the development of novel microscopy approaches. Introduction to Fluorescence helps readers acquire a sound understanding of basic fluorescence theory and practice. It describes general principles in a straightforward way and uses examples from a variety of disciplines to demonstrate them. In color throughout, the book takes readers through the history of important discoveries to the most current advances. It introduces the fundamentals of the fluorescence phenomenon and gives detailed examples of fluorescence applications in the molecular life sciences, including biochemistry, biophysics, clinical chemistry and diagnostics, pharmaceutical science, and cell and molecular biology. The author presents the basic theories underlying the applications and offers in-depth information on practical aspects. Along with a list of references in each chapter, the text incorporates more than 250 figures that clearly illustrate the concepts and gives the chemical structures of the most widely used fluorescent molecules. In addition, the appendix provides a "Rogue’s Gallery" of the most common errors and pitfalls to avoid.

Fluorescence Microscopy

Author: F. W. D. Rost

Publisher: Cambridge University Press

ISBN: 9780521410885

Category: Science

Page: 473

View: 7275

Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts, for example in living cells. This magnificent new work provides comprehensive cover of all aspects of fluorescence microscopy - including instrumentation, applications, and the history of the technique. The first volume deals with instrumentation and techniques for fluorescence microscopy, and includes a chapter on quantification and scanning. The second volume deals with the applications of fluorescence microscopy in many fields. It includes information on autofluorescence, and an invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations.

Principles of Fluorescence Spectroscopy

Author: Joseph R. Lakowicz

Publisher: Springer Science & Business Media

ISBN: 9780387463124

Category: Science

Page: 954

View: 6107

The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.


Gems and Minerals Under Ultraviolet Light

Author: Manuel Robbins

Publisher: N.A


Category: Nature

Page: 374

View: 2857

The complete source for understanding and locating fluorescent minerals. Documents all major locations in the U.S. and throughout the world. One of very few publications on this subject. Includes experiments for home and school use.

Reviews in Fluorescence 2005

Author: Chris D. Geddes,Joseph R. Lakowicz

Publisher: Springer Science & Business Media

ISBN: 9780387236902

Category: Science

Page: 444

View: 2327

Last year we launched Volume 1 of the Reviews in Fluorescence series. The volume was well-received by the fluorescence community, with many e-mails and letters providing valuable feedback, we subsequently thank you all for your continued support. After the volume was published we were most pleased to learn that the volume is to be citable and indexed, appearing on the ISI database. Subsequently, as well as the series having an impact number in due course, individual chapters will appear on the database and be both citable and keyword searchable. We feel that this will be a powerful resource to both authors and readers, further disseminating leading-edge fluorescence based material. Our intention with this new series is to both disseminate and archive the most recent developments in both past and emerging fluorescence based disciplines. While all chapters are invited, we welcome and indeed encourage the fluorescence community to suggest areas of interest that they feel need to be covered by the series. In this new volume. Reviews in Fluorescence 2005, Volume 2, we have invited reviews in areas such as: Multi-dimensional Time-correlated Single Photon Counting; Fluorescence Correlation Spectroscopy; RNA folding; Lanthanide Probes and Fluorescent Biosensors to name but just a few. We hope you find this volume a useful resource and we look forward to receiving any suggestions you may have. Finally we would like to thank the authors for their timely articles, Caroleann Aitken for the fi-ont cover design, Kadir Asian for typesetting and Mary Rosenfeld for administrative support.

Fluorescence Microscopy and Fluorescent Probes

Author: J. Slavík

Publisher: Springer Science & Business Media

ISBN: 1489918663

Category: Science

Page: 306

View: 2158

Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.

Structure and Dynamics of Macromolecules: Absorption and Fluorescence Studies

Author: J.R. Albani

Publisher: Elsevier

ISBN: 9780080474489

Category: Science

Page: 426

View: 1781

Structure and Dynamics of Macromolecules: Absorption and Fluorescence Studies is clearly written and contains invaluable examples, coupled with illustrations that demonstrate a comprehensible analysis and presentation of the data. This book offers practical information on the fundamentals of absorption and fluorescence, showing that it is possible to interpret the same result in different ways. It is an asset to students, professors and researchers wishing to discover or use absorption and fluorescence spectroscopy, and to scientists working on the structure and dynamics of macromolecules. * Offers concise information on the fundamentals of absorption and fluorescence * Critically reviews examples taken from previously published literature * Highly illustrated, it is suitable for academic and institutional libraries and government laboratories

Fluorescence Microscopy

From Principles to Biological Applications

Author: Ulrich Kubitscheck

Publisher: John Wiley & Sons

ISBN: 3527338373

Category: Medical

Page: 504

View: 569

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Fluorescence Microscopy

Author: Brian Herman

Publisher: Bios Scientific Pub Limited

ISBN: 9780387915517

Category: Science

Page: 170

View: 4396

Fluoresence Microscopy is the revised, updated and expanded second edition of a best-selling introductory text on the use of fluorescence microscopy in cell biology. The book covers the fundamental principles of fluorescence and their application to fluorescence microscopy. A detailed description of the fluorescence microscope and other required equipment is given, too. Applications to immunofluorescence, in situ hybridization, and photomicrography are presented in full, practical detail. Troubleshooting guidance is provided, along with many practical hints, to guide the user through commonly encountered problems.

Reviews in Fluorescence 2007

Author: Chris D Geddes

Publisher: Springer Science & Business Media

ISBN: 9780387887227

Category: Science

Page: 400

View: 9861

This fourth volume in the Springer series summarizes the year’s progress in fluorescence, with authoritative analytical reviews specialized enough for professional researchers, yet also appealing to a wider audience of scientists in related fields.

Fluorescence Spectroscopy in Biology

Advanced Methods and their Applications to Membranes, Proteins, DNA, and Cells

Author: Martin Hof,Rudolf Hutterer,V. Fidler

Publisher: Springer Science & Business Media

ISBN: 3540270043

Category: Science

Page: 305

View: 1416

Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.

Introduction to fluorescence microscopy

Author: J. S. Ploem,H. J. Tanke

Publisher: Oxford University Press

ISBN: 9780198564089

Category: Science

Page: 56

View: 8899

This handbook provides a thorough and detailed introduction to fluorescence microscopy by exploring three main areas of the field. First discussed are the physical principles of the phenomenon of fluorescence and fluorescence microscopy, including transmitted versus incident illumination. Next is a detailed description of various microscope parts, including lenses, filters, and light sources, and a discussion of the main applications of fluorescence microscopy, including immunofluorescence, microflurometry, and photomicrography. The final section enables the reader to solve the most common problems in fluorescence microscopy. Each chapter includes an up-to-date list of review articles and books for further reading, and there are illustrations throughout the text of typical microscopes and optical components. Biologists, students, and researchers of fluorescence microscopy will value this clear and indispensable guide to the field.

Topics in Fluorescence Spectroscopy

Volume 4: Probe Design and Chemical Sensing

Author: Joseph R. Lakowicz

Publisher: Springer Science & Business Media

ISBN: 9780306447846

Category: Science

Page: 502

View: 9779

Time-resolved fluorescence spectroscopy is widely used as a research tool in bioch- istry and biophysics. These uses of fluorescence have resulted in extensive knowledge of the structure and dynamics of biological macromolecules. This information has been gained by studies of phenomena that affect the excited state, such as the local environment, quenching processes, and energy transfer. Topics in Fluorescence Spectroscopy, Volume 4: Probe Design and Chemical Sensing reflects a new trend, which is the use of time-resolved fluorescence in analytical and clinical chemistry. These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. For instance, pho- multiplier tubes (PMT) were previously bulky devices. Miniature PMTs are now available, and the performance of simpler detectors is continually improving. There is also considerable effort to develop fluorophores that can be excited with the red/ne- infrared (NIR) output of laser diodes. Using such probes, one can readily imagine small time-resolved fluorometers, even hand-held devices, being used fordoctor’s office or home health care.

Introduction to Fluorescence Sensing

Author: Alexander P. Demchenko

Publisher: Springer Science & Business Media

ISBN: 140209003X

Category: Medical

Page: 590

View: 3221

Fluorescence sensing is a rapidly developing field of research and technology. Its target is nearly the whole world of natural and synthetic compounds being detected in different media including living bodies. The application area range from control of industrial processes to environment monitoring and clinical diagnostics. Among different detection methods fluorescence techniques are distinguished by ultimate sensitivity, high temporal and spatial resolution and versatility that allows not only remote detection of different targets but their imaging within the living cells. The basic mechanism of sensing is the transmission of the signal produced by molecular interaction with the target to fluorescent molecules, nanoparticles and nanocomposites with the detection by devices based on modern electronics and optics. In this interdisciplinary field of research and development the book is primarily intended to be a guide for students and young researchers. It is also addressed to professionals involved in active research and product development serving as a reference for the recent achievements. The users of these products will find description of principles that could allow proper selection of sensors for particular needs. Making a strong link between education, research and product development, this book discusses future directions.

New Trends in Fluorescence Spectroscopy

Applications to Chemical and Life Sciences : With 187 Figures and 39 Tables

Author: Bernard Valeur

Publisher: Springer Science & Business Media

ISBN: 9783540677796

Category: Science

Page: 490

View: 7056

This first volume in the new "Springer Series on Fluorescence" brings together fundamental and applied research from this highly interdisciplinary field ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of state-of-the-art articles will serve as an invaluable tool for practitioners and novices and provide inspiration for new developments and applications.

Practical Fluorescence, Second Edition

Author: George G. Guilbault

Publisher: CRC Press

ISBN: 9780824783501

Category: Science

Page: 826

View: 1599

New edition (first, 1973) of an introduction to the principles and applications of all phases of luminescence spectroscopy. Contains (all rewritten) chapters on general aspects of luminescence, instrumentation, effects of molecular structure and environment, inorganic analysis, phosphorescence, fluo

Applied Fluorescence in Chemistry, Biology and Medicine

Author: Wolfgang Rettig,Bernd Strehmel,Sigurd Schrader,Holger Seifert

Publisher: Springer Science & Business Media

ISBN: 3642599036

Category: Science

Page: 562

View: 2956

This interdisciplinary book gives a comprehensive survey of the state-of-the-art: from applications and trends in fluorescence techniques in science to medicine and engineering. Written for practitioners and researchers in industry and academia, it covers fields like environmental and materials science, biology, medicine, physics and chemistry. Moreover, it reports on such new and breathtaking methods as ultra-fast time-resolved or single molecule spectroscopy, gives examples of applications in the fields of electroluminescent polymers, visualization of membrane potentials in neurons and fluorescence imaging of the brain.

Quantitative Fluorescence Microscopy

Author: F. W. D. Rost

Publisher: Cambridge University Press

ISBN: 9780521394222

Category: Medical

Page: 236

View: 9825

Quantitative fluorescence microscopy is concerned with making measurements from fluorescent specimens in a fluorescence microscope, by measuring fluorescence emission from a defined area or areas of a specimen. This technique is most commonly used to determine the amount of some specific substance, such as DNA, in some particular area of a cell. But it has many other uses; for example, it can be used to identify certain substances in the cell by examining their fluorescence characteristics. This book is a complete guide to this technique for all biologists. It describes the principles and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. There is also a discussion of the exciting developments in confocal fluorescence microscopy which allows the three dimensional distribution of particular substances to be determined. Everyone presently using this technique, or wishing to start using it will need to read this book.

Advanced Concepts in Fluorescence Sensing

Part A: Small Molecule Sensing

Author: Chris D. Geddes,Joseph R. Lakowicz

Publisher: Springer Science & Business Media

ISBN: 9780387233345

Category: Science

Page: 327

View: 2103

Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology. Complementary volumes 9 and 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.