Fluorescence Microscopy

Author: F. W. D. Rost

Publisher: Cambridge University Press

ISBN: 9780521410885

Category: Science

Page: 473

View: 5267

Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts, for example in living cells. This magnificent new work provides comprehensive cover of all aspects of fluorescence microscopy - including instrumentation, applications, and the history of the technique. The first volume deals with instrumentation and techniques for fluorescence microscopy, and includes a chapter on quantification and scanning. The second volume deals with the applications of fluorescence microscopy in many fields. It includes information on autofluorescence, and an invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations.

Introduction to Fluorescence

Author: David M. Jameson

Publisher: Taylor & Francis

ISBN: 1439806047

Category: Science

Page: 313

View: 6120

The phenomenon known as fluorescence is now widely used in the chemical and life sciences largely due to the development of highly sophisticated fluorescent probe chemistries and the commercial availability of these probes as well as the development of novel microscopy approaches. Introduction to Fluorescence helps readers acquire a sound understanding of basic fluorescence theory and practice. It describes general principles in a straightforward way and uses examples from a variety of disciplines to demonstrate them. In color throughout, the book takes readers through the history of important discoveries to the most current advances. It introduces the fundamentals of the fluorescence phenomenon and gives detailed examples of fluorescence applications in the molecular life sciences, including biochemistry, biophysics, clinical chemistry and diagnostics, pharmaceutical science, and cell and molecular biology. The author presents the basic theories underlying the applications and offers in-depth information on practical aspects. Along with a list of references in each chapter, the text incorporates more than 250 figures that clearly illustrate the concepts and gives the chemical structures of the most widely used fluorescent molecules. In addition, the appendix provides a "Rogue’s Gallery" of the most common errors and pitfalls to avoid.

Fluorescence Microscopy and Fluorescent Probes

Author: Jan Slav’k

Publisher: Springer Science & Business Media

ISBN: 9780306460210

Category: Science

Page: 272

View: 9501

The April 1997 conference held in Prague attracted the cream of primarily European and Russian researchers (with a handful from the US, primarily from the U. of Maryland School of Medicine) to the burgeoning biological and medical applications of innovative optical technology, particularly laser con

Reviews in Fluorescence 2005

Author: Chris D. Geddes,Joseph R. Lakowicz

Publisher: Springer Science & Business Media

ISBN: 9780387236902

Category: Science

Page: 444

View: 8182

Last year we launched Volume 1 of the Reviews in Fluorescence series. The volume was well-received by the fluorescence community, with many e-mails and letters providing valuable feedback, we subsequently thank you all for your continued support. After the volume was published we were most pleased to learn that the volume is to be citable and indexed, appearing on the ISI database. Subsequently, as well as the series having an impact number in due course, individual chapters will appear on the database and be both citable and keyword searchable. We feel that this will be a powerful resource to both authors and readers, further disseminating leading-edge fluorescence based material. Our intention with this new series is to both disseminate and archive the most recent developments in both past and emerging fluorescence based disciplines. While all chapters are invited, we welcome and indeed encourage the fluorescence community to suggest areas of interest that they feel need to be covered by the series. In this new volume. Reviews in Fluorescence 2005, Volume 2, we have invited reviews in areas such as: Multi-dimensional Time-correlated Single Photon Counting; Fluorescence Correlation Spectroscopy; RNA folding; Lanthanide Probes and Fluorescent Biosensors to name but just a few. We hope you find this volume a useful resource and we look forward to receiving any suggestions you may have. Finally we would like to thank the authors for their timely articles, Caroleann Aitken for the fi-ont cover design, Kadir Asian for typesetting and Mary Rosenfeld for administrative support.

Handbook of Fluorescence Spectroscopy and Imaging

From Ensemble to Single Molecules

Author: Markus Sauer,Johan Hofkens,J?rg Enderlein

Publisher: John Wiley & Sons

ISBN: 3527633529

Category: Science

Page: 280

View: 2684

Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

Principles of Fluorescence Spectroscopy

Author: Joseph R. Lakowicz

Publisher: Springer Science & Business Media

ISBN: 9780387463124

Category: Science

Page: 954

View: 3207

The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

New Trends in Fluorescence Spectroscopy

Applications to Chemical and Life Sciences

Author: Bernard Valeur,Jean-Claude Brochon

Publisher: Springer Science & Business Media

ISBN: 364256853X

Category: Science

Page: 490

View: 874

This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.

Fluorescence

Poems

Author: Jennifer K. Dick

Publisher: University of Georgia Press

ISBN: 9780820326917

Category: Poetry

Page: 89

View: 5139

In Jennifer K. Dick's first book, Fluorescence, very real places--Paris, Massachusetts, Colorado, Iowa, Morocco--mix into the imagined, into Breughelian villages where there's "a persimmon in the corner knitting." These places are inhabited by varied but always very real bodies, stretching outward from their own edges and encountering, or engendering, a certain luminescence in the process. What happens when we exceed ourselves? When fragments of dream are lifted to the surface and through to something beyond? Clues, keys, indications--all that once seemed certain slips off into code. These poems use language to crack it.

Fluorescence Applications in Biotechnology and Life Sciences

Author: Ewa M. Goldys

Publisher: John Wiley & Sons

ISBN: 0470083700

Category: Science

Page: 367

View: 8151

Fluorescence Applications in Biotechnology and the Life Sciences Edited by Ewa M. Goldys A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences Fluorescence Applications in Biotechnology and the Life Sciences is the first reference in this important subject area to focus specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It is designed to raise awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environ-mental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book will appeal to postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.

Fluorescence

Gems and Minerals Under Ultraviolet Light

Author: Manuel Robbins

Publisher: N.A

ISBN: N.A

Category: Nature

Page: 374

View: 8855

The complete source for understanding and locating fluorescent minerals. Documents all major locations in the U.S. and throughout the world. One of very few publications on this subject. Includes experiments for home and school use.

Reviews in Fluorescence 2007

Author: Chris D Geddes

Publisher: Springer Science & Business Media

ISBN: 9780387887227

Category: Science

Page: 400

View: 9284

This fourth volume in the Springer series summarizes the year’s progress in fluorescence, with authoritative analytical reviews specialized enough for professional researchers, yet also appealing to a wider audience of scientists in related fields.

Fluorescence Spectroscopy in Biology

Advanced Methods and their Applications to Membranes, Proteins, DNA, and Cells

Author: Martin Hof,Rudolf Hutterer,V. Fidler

Publisher: Springer Science & Business Media

ISBN: 9783540223382

Category: Science

Page: 305

View: 8486

Topics in Fluorescence Spectroscopy

Volume 4: Probe Design and Chemical Sensing

Author: Joseph R. Lakowicz

Publisher: Springer Science & Business Media

ISBN: 9780306447846

Category: Science

Page: 502

View: 912

Time-resolved fluorescence spectroscopy is widely used as a research tool in bioch- istry and biophysics. These uses of fluorescence have resulted in extensive knowledge of the structure and dynamics of biological macromolecules. This information has been gained by studies of phenomena that affect the excited state, such as the local environment, quenching processes, and energy transfer. Topics in Fluorescence Spectroscopy, Volume 4: Probe Design and Chemical Sensing reflects a new trend, which is the use of time-resolved fluorescence in analytical and clinical chemistry. These emerging applications of time-resolved fluorescence are the result of continued advances in laser detector and computer technology. For instance, pho- multiplier tubes (PMT) were previously bulky devices. Miniature PMTs are now available, and the performance of simpler detectors is continually improving. There is also considerable effort to develop fluorophores that can be excited with the red/ne- infrared (NIR) output of laser diodes. Using such probes, one can readily imagine small time-resolved fluorometers, even hand-held devices, being used fordoctor’s office or home health care.

Introduction to Fluorescence Sensing

Author: Alexander P. Demchenko

Publisher: Springer Science & Business Media

ISBN: 140209003X

Category: Medical

Page: 590

View: 4295

Fluorescence sensing is a rapidly developing field of research and technology. Its target is nearly the whole world of natural and synthetic compounds being detected in different media including living bodies. The application area range from control of industrial processes to environment monitoring and clinical diagnostics. Among different detection methods fluorescence techniques are distinguished by ultimate sensitivity, high temporal and spatial resolution and versatility that allows not only remote detection of different targets but their imaging within the living cells. The basic mechanism of sensing is the transmission of the signal produced by molecular interaction with the target to fluorescent molecules, nanoparticles and nanocomposites with the detection by devices based on modern electronics and optics. In this interdisciplinary field of research and development the book is primarily intended to be a guide for students and young researchers. It is also addressed to professionals involved in active research and product development serving as a reference for the recent achievements. The users of these products will find description of principles that could allow proper selection of sensors for particular needs. Making a strong link between education, research and product development, this book discusses future directions.

Practical Fluorescence, Second Edition

Author: George G. Guilbault

Publisher: CRC Press

ISBN: 9780824783501

Category: Science

Page: 826

View: 2667

New edition (first, 1973) of an introduction to the principles and applications of all phases of luminescence spectroscopy. Contains (all rewritten) chapters on general aspects of luminescence, instrumentation, effects of molecular structure and environment, inorganic analysis, phosphorescence, fluo

Introduction to fluorescence microscopy

Author: J. S. Ploem,H. J. Tanke

Publisher: Oxford University Press

ISBN: 9780198564089

Category: Science

Page: 56

View: 5472

This handbook provides a thorough and detailed introduction to fluorescence microscopy by exploring three main areas of the field. First discussed are the physical principles of the phenomenon of fluorescence and fluorescence microscopy, including transmitted versus incident illumination. Next is a detailed description of various microscope parts, including lenses, filters, and light sources, and a discussion of the main applications of fluorescence microscopy, including immunofluorescence, microflurometry, and photomicrography. The final section enables the reader to solve the most common problems in fluorescence microscopy. Each chapter includes an up-to-date list of review articles and books for further reading, and there are illustrations throughout the text of typical microscopes and optical components. Biologists, students, and researchers of fluorescence microscopy will value this clear and indispensable guide to the field.

Quantitative Fluorescence Microscopy

Author: F. W. D. Rost

Publisher: Cambridge University Press

ISBN: 9780521394222

Category: Medical

Page: 236

View: 1830

Quantitative fluorescence microscopy is concerned with making measurements from fluorescent specimens in a fluorescence microscope, by measuring fluorescence emission from a defined area or areas of a specimen. This technique is most commonly used to determine the amount of some specific substance, such as DNA, in some particular area of a cell. But it has many other uses; for example, it can be used to identify certain substances in the cell by examining their fluorescence characteristics. This book is a complete guide to this technique for all biologists. It describes the principles and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. There is also a discussion of the exciting developments in confocal fluorescence microscopy which allows the three dimensional distribution of particular substances to be determined. Everyone presently using this technique, or wishing to start using it will need to read this book.