GFP Whole Cell Microbial Biosensors

Scale-up and scale-down effects on biopharmaceutical processes

Author: Frank Delvigne

Publisher: Momentum Press

ISBN: 1606504177

Category: Technology & Engineering

Page: 52

View: 8002

Two strategies are usually considered for the optimization of microbial bioprocesses. The first one involves genetic or metabolic engineering of the target microbial strains in order to improve its production efficiency or its tolerance to adverse conditions. The second one is based on the chemical engineering improvement of the bioreactors and scaling-up rules. This work is more particularly dedicated to this second class of parameters. Recent developments in bioreactor technologies follow the scaling-out principle, i.e. carrying out several cultures in parallel with controlled conditions for screening purposes. Several mini-bioreactor concepts, i.e. bioreactor with working volume of 1 to 100 mL with controlling devices, have been developed following this principle. In general, chemical engineering similarities between conventional stirred bioreactors and their miniature equivalent are well characterized. However, the actual scaling-up rules are not able to cope with the complexity of the microbial stress response. Indeed, microbial stress response still remains not completely understood considering the process perturbations and the environmental fluctuations accompanying the scaling-up to industrial bioreactors. At this time, this kind of response can only be experimentally predicted by using scale-down bioreactors, i.e. lab-scale bioreactors designed in order to reproduce mixing imperfections that have to be expected at large-scale. However, the use of such an approach is time consuming and requires an experimented staff to elaborate the scaling-down protocols. Indeed, bioprocess development involves several steps which cannot be necessarily linked with each other considering the different cultivation equipment used.

Bioluminescent Microbial Biosensors

Design, Construction, and Implementation

Author: Gerald Thouand,Robert S. Marks

Publisher: CRC Press

ISBN: 9814613665

Category: Medical

Page: 236

View: 1796

This book describes the design and the use of bioluminescent biosensors. It introduces beginners and experienced researchers starting in the microbiological biosensor domain to the practical aspects of building a luminescent microbial biosensor. It is also a source of information about other applications that use microbial cells. Each chapter focuses as far as possible on the technological conception of the presented biosensor with a clear demonstration of the issues in the design and how to reach the proof of concept. The book is divided into three practical sections facilitating the reader to easily access the information, starting from the bioreporter handling (free, immobilized, or spore) to the engineering of the measurement platform (fiber optic, CCD, lensless platform, free-cell bioreactor, CD platform).

Bioluminescence & Chemiluminescence

Progress & Current Applications

Author: Larry J. Kricka,Philip E. Stanley

Publisher: World Scientific

ISBN: 9789812776624

Category: Science

Page: 556

View: 810

The Proceedings of the 12th International Symposium on Bioluminescence (BL) and Chemiluminescence (CL) contains up-to-date information on the latest developments in BL and CL presented by scientists from around the world.Light-emitting reactions are now a vital component of many key technologies in research and in routine analytical laboratories OCo replacing radionuclides in many situations. This volume presents a compilation of the latest developments from key experts and leading-edge researchers in this area."

Luminescent Proteins—Advances in Research and Application: 2012 Edition

Author: N.A

Publisher: ScholarlyEditions

ISBN: 1464992886

Category: Science

Page: 498

View: 9151

Luminescent Proteins—Advances in Research and Application: 2012 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Luminescent Proteins. The editors have built Luminescent Proteins—Advances in Research and Application: 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Luminescent Proteins in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Luminescent Proteins—Advances in Research and Application: 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Applications of Microbial Engineering

Author: Vijai Kumar Gupta,Monika Schmoll,Minna Maki,Maria Tuohy,Marcio Antonio Mazutti

Publisher: CRC Press

ISBN: 1466585773

Category: Medical

Page: 504

View: 8527

The microbial engineering technologies have been identified as an essential and important subject area of engineering and applied biological sciences. A microbial engineer works on the biological, chemical and engineering aspects of biotechnology, manipulating microbes and developing new uses for microbes. In agriculture, bioprocess engineering, in biotechnology, genetic engineering, microbial vaccines, and the development of bionanotechnology, microbial engineering could be recognized as high potential technologies in the current scenario for economic development. Scientists and engineers are motivated for sustainable green technology as a part of an upcoming industrial revolution turning more and more to processes involving microorganisms. Applications of Microbial Engineering provides a better understanding of industrially important genetically manipulated and engineered prokaryotic and eukaryotic cell systems. The content of this book are based on most recent developments in microbial engineering. The contributions by specialists on the respective topics provide a profound scientific basis for further research. It is expected that this book will be a valuable resource for researchers as well as students dealing with microbiology and biotechnology.

Whole Cell Sensing System II

Applications

Author: Shimshon Belkin,Man Bock Gu

Publisher: Springer Science & Business Media

ISBN: 3642128521

Category: Science

Page: 214

View: 3504

Applications: - Applications of Microbial Cell Sensors, by Mifumi Shimomura-Shimizu and Isao Karube - Whole-Cell Bioreporters for the Detection of Bioavailable Metals, by Anu Hynninen and Marko Virta - Bacteriophage-Based Pathogen Detection, by Steven Ripp - Cell-Based Genotoxicity Testing, by Georg Reifferscheid and Sebastian Buchinger - Cytotoxicity and Genotoxicity Reporter Systems Based on the Use of Mammalian Cells, by Christa Baumstark-Khan, Christine E. Hellweg, and Günther Reitz - Live Cell Optical Sensing for High Throughput Applications, by Ye Fang - Cyanobacterial Bioreporters as Sensors of Nutrient Availability, by George S. Bullerjahn, Ramakrishna Boyanapalli, Mark J. Rozmarynowycz, and R. Michael L. McKay - Application of Microbial Bioreporters in Environmental Microbiology and Bioremediation, by E. E. Diplock , H. A. Alhadrami , and G. I. Paton

Recognition Receptors in Biosensors

Author: Mohammed Zourob

Publisher: Springer Science & Business Media

ISBN: 9781441909190

Category: Technology & Engineering

Page: 863

View: 4736

Recognition receptors play a key role in the successful implementation of chemical and biosensors. Molecular recognition refers to non-covalent speci?c binding between molecules, one of which is typically a macromolecule or a molecular assembly, and the other is the target molecule (ligand or analyte). Biomolecular recognition is typically driven by many weak interactions such as hydrogen bo- ing, metal coordination, hydrophobic forces, van der Waals forces, pi-pi interactions and electrostatic interaction (due to permanent charges, dipoles, and quadrupoles) the polarization of charge distributions by the interaction partner leading to ind- tion and dispersion forces, and Pauli-exclusion-principle-derived inter-atomic repulsion, and a strong, “attractive” force arising largely from the entropy of the solvent and termed the hydrophobic effect. In recent years, there has been much progress in understanding the forces that drive the formation of such complexes, and how these forces are relate to the physical properties of the interacting molecules and their environment allows rational design of molecules and materials that interact in speci?c and desired ways. This book presents a signi?cant and up-to-date review of the various recognition elements, their immobilization, characterization techniques by a panel of dist- guished scientists. This work is a comprehensive approach to the recognition receptors area presenting a thorough knowledge of the subject and an effective integration of these receptors on sensor surfaces in order to appropriately convey the state-of the-art fundamentals and applications of the most innovative approaches.

Biosensoren

Author: Elizabeth A.H. Hall

Publisher: Springer-Verlag

ISBN: 364278660X

Category: Science

Page: 417

View: 2309

Biosensoren vereinigen so inkompatible Welten wie Molekularbiologie und Elektronik. Das Potential für Anwendungen von Biosensoren ist enorm hoch und reicht von der Messung der Ölverschmutzung unserer Weltmeere bis zur Bestimmung des Glucosespiegels im Blut. Das vorliegende Buch gibt zunächst eine Einführung in die Molekularbiologie und Biochemie, soweit dies zum Verständnis der Biosensor-Technologie notwendig ist. Es werden dann die verschiedensten Biosensor-Typen sowie deren wissenschaftliche und kommerzielle Anwendungen beschrieben.

Applications of Cell Immobilisation Biotechnology

Author: Viktor Nedovic,Ronnie Willaert

Publisher: Springer Science & Business Media

ISBN: 9781402032295

Category: Science

Page: 573

View: 2373

Cell immobilisation biotechnology is a multidisciplinary area, shown to have an important impact on many scientific subdisciplines – including biomedicine, pharmacology, cosmetology, food and agricultural sciences, beverage production, industrial waste treatment, analytical applications, biologics production. "Cell Immobilisation Biotechnology" is an outcome of the editors’ intention to collate the extensive and widespread information on fundamental aspects and applications of immobilisation/encapsulation biotechnology into a comprehensive reference work and to provide an overview of the most recent results and developments in this domain. "Cell Immobilisation Biotechnology" is divided into the two book volumes, FOBI 8A and FOBI 8B. The FOBI 8A volume, Fundamentals of Cell Immobilisation Biotechnology, is dedicated to fundamental aspects of cell immobilisation while the present volume, FOBI 8B, Applications of Cell Immobilisation Biotechnology, deals with diverse applications of this technology.

Nucleic Acids and Proteins in Soil

Author: Paolo Nannipieri,Kornelia Smalla

Publisher: Springer Science & Business Media

ISBN: 354029449X

Category: Science

Page: 458

View: 8268

With millions of different bacterial species living in soil, the microbial community is extremely complex, varying at very small scales. Microbe-driven functions are essential for most processes in soil. Thus, a better understanding of this microbial diversity will be invaluable for the management of the various soil functions. Nucleic Acids and Proteins in Soil combines traditional approaches in soil microbiology and biochemistry with the latest techniques in molecular microbial ecology. Included are methods to analyse the presence and importance of nucleic acids and proteins both inside and outside microbial cells, the horizontal gene transfer which drives bacterial diversity, as well as soil proteomes. Further chapters describe techniques such as PCR, fingerprinting, the challenging use of gene arrays for structural and functional analysis, stable isotope probing to identify in situ metabolic functions, and the use of marker and reporter genes in soil microbial ecology.

Environmental Chemistry

Green Chemistry and Pollutants in Ecosystems

Author: Eric Lichtfouse

Publisher: Springer Science & Business Media

ISBN: 9783540228608

Category: Nature

Page: 780

View: 6523

Environmental chemistry is a new, fast developing science aimed at deciphering fundamental mechanisms ruling the behaviour of pollutants in ecosystems. Applying this knowledge to current environmental issues leads to the remediation of environmental media, and to new, low energy, low emission, sustainable processes. This book describes the state-of-the-art advances regarding the pollution of water, soils, atmosphere, food and living organisms by toxic metals, fossil fuels, pesticides and other organic pollutants. Furthermore, the eco-toxicology section presents novel bio-assays to assess the toxicity of various pollutants such as dioxins and endocrine disrupters within complex media. The green chemistry section highlights novel chemical reactions based upon environmentally friendly conditions. The analytical chemistry section describes very sensitive methods which trace the fate of pollutants in complex ecosystems.

Molecular Microbial Ecology

Author: A. Mark Osborn,Cindy Jane Smith

Publisher: Garland Science

ISBN: 9781859962831

Category: Science

Page: 381

View: 6949

Microoganisms are distributed across every ecosystem, and microbial transformations are fundamental to the operation of the biosphere. Microbial ecology is the study of this interaction between microorganisms and their environment, and arguably represents one of the most important areas of biological research. Yet for many years our study of microbial flora was severely limited: the primary method of culturing microorganisms on media allowed us to study only between 0.1 and 10% of the total microbial flora in any given environment. Molecular Microbial Ecology gives a comprehensive guide to the recent revolution in the study of microorganisms in the environment. Details are given on molecular methods for isolating some of the previously uncultured and numerically dominant microbial groups. PCR-based approaches to studying prokaryotic systematics are described, including ribosomal RNA analysis and stable isotope probing. Later chapters cover DNA hybridisation techniques (including fluorescent in situ hybridisation), as well as genomic and metagenomic approaches to microbial ecology. Gathering together some of the world's leading experts, this book provides an invaluable introduction to the modern theory and molecular methods used in studying microbial ecology.

Chemistry of Marine Water and Sediments

Author: Antonio Gianguzza,Ezio Pelizzetti,Silvio Sammartano

Publisher: Springer Science & Business Media

ISBN: 9783540420552

Category: Nature

Page: 508

View: 4506

The book deals with the processes in marine environment with particular emphasis on the interface processes (sediments- water and atmosphere-water) regarding organic matter and energy fluxes, carbon dioxide intake and transformation. Particular analytical methodologies concerning biosensors for analysis in situ are discussed.

Bioavailability of Organic Xenobiotics in the Environment

Practical Consequences for the Environment

Author: P. Baveye,J.-C. Block,Vladislav Vladimirovich Goncharuk

Publisher: Springer Science & Business Media

ISBN: 9780792359449

Category: Medical

Page: 503

View: 8807

Proceedings of the NATO Advanced Study Institute, held in Prague, Czech Republic from 18-29 August 1997

Optical Sensor Systems in Biotechnology

Author: Govind Rao

Publisher: Springer Science & Business Media

ISBN: 3642034705

Category: Science

Page: 162

View: 4063

Of all things natural, light is the most sublime. From the very existential belief of the origins of the universe to its role in the evolution of life on earth, light has been inextricably woven into every aspect of our lives. I am grateful to Springer-Verlag and Thomas Scheper for this invitation to organize this volume that continues to expand the use of light to create next generation sensing applications. Indeed, the very act of expanding the frontiers of learning and knowledge are referred to in many languages and cultures as enlightenment. Early optical instruments relied largely on simple combinations of mirrors, prisms and lenses. With these simple devices, substantial progress was made in our understanding of the properties of light and of its interactions with matter. Things got more complicated with the evolution of optical instruments in labo- tory use. Early systems used bulky and expensive hardware to generate light, split it into the desired wavelengths and finally collect it for analysis. The discovery of the laser pushed the technology further, but did not do much to make its adoption more widespread as the lasers themselves were large and required substantial el- trical power to operate. The true revolution is just beginning. Advances in mic- electronics have resulted in the possibility of truly low-cost (using the consumer electronics industry as a parallel) devices that exploit optical measurement technology.

Advanced Environmental Sensing Technology II

31 October-1 November, 2001, Newton, [Massachusetts] USA

Author: Dinh Tuan Vo,Stephanus Büttgenbach,Society of Photo-Optical Instrumentation Engineers

Publisher: Society of Photo Optical

ISBN: N.A

Category: Technology & Engineering

Page: 266

View: 2627